Product Name VITAMIN K1（PHYTONADIONE）

CAS No.  84-80-0

Appearance    Yellow to orange clarified viscous liquid; odorless or almost odorless; easy to decompose when exposed to light.soluble in chloroform, ether or vegetable oil, slightly soluble in ethanol and insoluble in water.

Isomer content %  ≤21

Refractive index  1.52-1.53

Identification 

 (1) Infrared Spectroscopy:197F (CN 1-May- 2020) 

(2)Ultraviolet-Visible Spectroscopy:197U (CN 1- May-2020) . Absorptivities do not differ by more than 3.0%

Limit of Menadione  No purple or blue color is produced

Menadione

Take 20mg of this product, add 2mL of trimethylpentane to dissolve, add ammonia test solution-ethanol (1:1) lmL and 2 drops of ethyl cyanoacetate, shake gently, after placing，the following layer of solution appears blue, Comparing with the trimethylpentane solution (containing 20 ug/mL of Menadione reference substance) 2 mL prepared by the same method, it should not be deeper.The test result less than 0.2%

Total Aerobic Microbial Colonies Count   CFU/g ≤1000

Yeasts & Molds  CFU/g ≤50

Vitamin K1 %   97—103 

Conclusion The product comply with USP 2021